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KMID : 0861020120270010051
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Korea Journal of Herbology
2012 Volume.27 No. 1 p.51 ~ p.58
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Effect on Acute reflux Esophagitis by Evodiae Fructus Aquous Extract
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Kim Dae-Jun
Roh Seong-Soo
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Abstract
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Objectives: This study was performed to investigate effect of evodiae fructus on acute reflux esophigitis rat
induced by pylorus and forestomach ligation operation.
Methods: Twenty-four laboratory rats were divided four groups and each group had six rats；normal intact
group, acute reflux esophagitis (RE) control group, two experiment RE group treated extract of evodiae fructus
600 mg/kg (EEF600) and 300 mg/kg (EEF300). All rats was fasted for 18 hr but free water, we induced RE by
pylorus and forestomach ligation operation. Intact group and RE control group rats were orally administered a
distilled water and two experiment groups were orally administed with EEF 600 mg/5ml/kg and 300 mg/5ml/kg.
One hour after, rats were anesthetized, intact group was cut the abdomen open and sutured with 2.0 silk
thread. RE control group and EEF group were cut the abdomen open, ligated pyloric canal and forestomach
with 2.0 silk thread and sutured. Six hour after the operation, rats were sacrified, collected bloods in the
abdominal vein, disectted a esophagus and stomach. The stomach was washed a 1 ml PBS and the esophagus
was cut longitudinally and pictured a innter mucosa area to research damages in esophagus.
Results: The esophagic tissue damage percentage of reflux esophagitis rat was increased compared to that of
normal intact group. But esophagic damage percentage of EEF 600 were significantly decreased compared to
that of RE control group. But there was no difference on gastric juice pH between control RE, alpha-tocopherol
administration rat group and EEF administration rat group. In esophagus of RE control rat, gastric damage
occurred severely and injury percentage of mucosa were increased, but EEF 600 mucous inflammatory damage
percentage was significantly compared to that of RE control group. Proinflammatory cytokines such as
TNF-alpha, IL-1beta and IL-6 in serum on RE control group were markedly grew than those of intact rat,
those of vechicle group treated with EEF 600 and EEF 300 were remarkably decreased compared to production
of proinflammatory cytokine of RE control group. In microscopic observation, intact group rat had no
hyperemia, mucous injury and exclusion, ulcer and edema. But it could showed mucosa damages, submucosa
edema and ulcer in RE control. However, administration of EEF 600 and EEF 300 made esophagus have less
inflammation and injury by gastric acid.
Conclusions: The results suggest that antiinflammatory Effect of EEF could attenuate the severity of reflux
esophagitis and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux
disease.
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KEYWORD
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Evodiae Fructus, reflux esophagitis, TNF-alpha, IL-1beta, Pylorus and forestomach ligation
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